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. 2020 Jul 9;10(19):8494–8512. doi: 10.7150/thno.44849

Figure 5.

Figure 5

LOXL4 is the direct target of miR-29b and miR-30d. (A) WT LOXL4 3'UTR and MUT LOXL4 3'UTR firefly luciferase activities were measured in MDA-MB-231 cells transfected with NC mimics or miR-29b mimics. (B) Putative binding site of miR-29b in LOXL4 3'UTR. (C) Luciferase reporter assays in MDA-MB-231 cells co-transfected with either WT LOXL4 3'UTR or MUT LOXL4 3'UTR 331 with the predicted miR-30d-binding site mutated and NC mimics or miR-30d mimics. (D) Putative binding site of miR-30d in LOXL4 3'UTR. (E) Western blotting of LOXL4 in MDA-MB-231 cells transfected with miR-29b/miR-30d mimics or miR-29b/miR-30d inhibitors. (F) qRT-PCR of LOXL4 and LOXL3 in MDA-MB-231 cells transfected with miR-29b/miR-30d mimics or miR-29b/miR-30d inhibitors. (G) qRT-PCR of miR-29b and LOXL4 in MDA-MB-231 cells stably expressing miR-29b and control cells. (H) qRT-PCR of miR-30d and LOXL4 in MDA-MB-231 cells stably expressing miR-30d and control cells.