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. 2020 Jul 9;10(19):8494–8512. doi: 10.7150/thno.44849

Figure 7.

Figure 7

EZH2 functions as a positive regulator of LOXL4 through epigenetically repressing miR-29b or miR-30d transcription. (A) qRT-PCR analysis of EZH2, LOXL4, and Dicer1 in MDA-MB-231 cells transfected with NC siRNA, EZH2 siRNA, Dicer1 siRNA, or EZH2 and Dicer1 siRNAs. (B) qRT-PCR analysis of miR-29b (left panel) and miR-30d (right panel) in MDA-MB-231 cells transfected with NC siRNA, EZH2 siRNA, Dicer1 siRNA, or EZH2 and Dicer1 siRNAs. (C) Immunoblotting analysis of EZH2, LOXL4, and Dicer1 in MDA-MB-231 cells transfected with NC siRNA, EZH2 siRNA, Dicer1 siRNA, or EZH2 and Dicer1 siRNAs. (D and E) qRT-PCR (D) and immunoblotting (E) analysis of LOXL4 and EZH2 in MDA-MB-231 cells transfected with NC siRNA, EZH2 siRNA, miR-29b inhibitor, or EZH2 siRNA and miR-29b inhibitor. (F and G) qRT-PCR (F) and immunoblotting (G) analysis of LOXL4 and EZH2 in MDA-MB-231 cells transfected with NC siRNA, EZH2 siRNA, miR-30d inhibitor, or EZH2 siRNA and miR-30d inhibitor.