Figure 9.

The EZH2-miR-29b/miR-30d-LOXL4 signaling pathway regulates macrophage infiltration and collagen remodeling in breast cancer. (A) Flow cytometry analysis of macrophage subpopulations of the 4T1 tumors in mice injected intraperitoneally with 4T1 cells treated with UNC1999. The percentages of (F4/80⁺) TAMs and (F4/80⁺CD206^high) M2-like TAMs were calculated. (B) Representative images of collagen fibrils from the 4T1 tumors in mice injected intraperitoneally with 4T1 cells treated with UNC1999. (C) Flow cytometry analysis of macrophage subpopulations of the 4T1 tumors in mice injected intraperitoneally with 4T1 cells overexpressing miR-29b or miR-30d. The percentages of (F4/80⁺) TAMs and (F4/80⁺CD206^high) M2-like TAMs were calculated. (D) Representative images of collagen fibrils from the 4T1 tumors in mice injected intraperitoneally with 4T1 cells overexpressing miR-29b or miR-30d. (E) F4/80⁺ TAMs were isolated from 4T1 tumors of mice injected intraperitoneally with 4T1 cells treated with UNC1999. M1 and M2 macrophage-associated gene expression were assessed by qRT-PCR. (F) F4/80⁺ TAMs were isolated from 4T1 tumors of mice injected intraperitoneally with 4T1 cells overexpressing miR-29b or miR-30d. M1 and M2 macrophage-associated gene expression was assessed by qRT-PCR. Scale bar: 30 µm.