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Effect of increased [Cl-]i on cellular mechanical properties. A-B) Normalized stiffness (G'/G0') of 16HBE14o- cells after treatment with buffers with various chloride concentrations and 1 h of treatment with CFTRinh-172 (10 µM) and IAA94 (40 µM). C) Stiffness of 16HBE14o- cells treated with high-KCl buffer, DMEM, and buffer with 25 mM [Cl-]i (resting [Cl-]i), for 1 h (n = 3 independent experiments, *P < 0.05; **P < 0.01 versus 25 mM). Data are presented as mean ± SD.
