Figure 2.
ZFP91 silencing promotes HCC cell growth, colony formation, migration and invasion in vitro and tumorigenesis and metastasis in vivo. (A-D) BEL7402 and SK-hep1 cells were transfected with anti-ZFP91 siRNAs; the indicated proteins (A), cell growth (B), migration and invasion (C), and colony formation (D) were determined. (E) The in vivo growth of the indicated cell lines stably silencing ZFP91 was examined. Mouse xenograft tumors are shown in the left panel. The weights of the xenograft tumors are presented in the right panel (n = 6). (F, G) Luc-labeled SK-hep1 cells (2 × 106 cells/mouse) were injected into NOD-SCID mice; the luciferase activity was visualized 2 months posttransplantation (n = 5) (F), and the metastatic tumor nodules in mouse lung were observed (G). Data are represented as mean ± SD.