KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Flag | Sigma-Aldrich | Cat# F3165; RRID:AB_259529 |
| Mouse monoclonal anti-Ubiquitin (P4D1) | Santa Cruz | Cat# Sc-8017; RRID:AB_2762364 |
| Mouse monoclonal anti-Pgk1 | Invitrogen | Cat# 459250; RRID:AB_2532235 |
| Mouse monoclonal anti-HA | Biolegend | Cat# 901515; RRID:AB_2565334 |
| Mouse anti-Flag M2 (agarose beads) affinity gel | Sigma | Cat#A2220; RRID:AB_10063035 |
| Mouse anti-GFP antibody | Santa Cruz | Cat# Sc-9966; RRID:AB_627235 |
| Secondary anti-mouse IgG HRP-linked antibody | Cell signaling | Cat# 7076; RRID:AB_330924 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| MG132 | Abeam | Ab141003 |
| Protease inhibitor cocktail set III | Millipore-Calbiochem | 539136 |
| Deubiquitinase inhibitor N-ethylmaleimide | Sigma | E3876 |
| Cycloheximide | Enzo Life Sciences | ALX380–269 G001 |
| ECL | PerkinElmer | NEL 104001 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: strain background W303; see Table S1 | This paper | N/A |
| Oligonucleotides | ||
|
SAN1 deletion forward primer ACTATAGATAGAAATTATTTAGCATTTCAGG ATAGTTCGTCGGATCCCCGGGTTAATTAA |
This paper | N/A |
|
SAN1 deletion reverse primer TGGATGACTGCCAATAGGACATATTTTCA TATTAACATACGAATTCGAGCTCGTTTAAAC |
This paper | N/A |
|
UBR1 deletion forward primer GTCCCTAATCTTTACAGGTCACACAAATT ACATAGAACATCGGATCCCCGGGTTAATTAA |
This paper | N/A |
|
UBR1 deletion reverse primer GTCAATCGACGCGCAAATGTTTAATAATGTAT AAGIIIIIGAATTCGAGCTCGTTTAAAC |
This paper | N/A |
|
HTA1-mApple forward primer AAAGAAGTCTGCCAAGGCTACCAAGGCTTC TCAAGAATTAGCGGCCGCTCTAGAACTAGTGG |
This paper | N/A |
|
HTA1-mApple reverse primer TTTAGTTCCTTCCGCCTTCTTTAAAATACCTGAA CCGATCCCCCCTCGAGGTCGACGGTATCG |
This paper | N/A |
| Software and Algorithms | ||
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |