(A) Quantification of urea synthesis by primary hepatocytes on soft, stiff and TCPS substrates; (B) quantification of albumin synthesis primary hepatocytes cultured on soft, stiff and TCPS substrates using ELISA; and (C) gene expression analysis albumin of primary hepatocytes cultured on soft, stiff and TCPS substrates on day 7, significant difference between the soft/stiff group and TCPS group is denoted by #p value < 0.05, ##p value < 0.005, significant difference between the soft and stiff group is denoted by *p value < 0.05.