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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Aug 6;1865(11):165527. doi: 10.1016/j.bbadis.2019.08.003

Table 1: Antibodies Used in Fluorescence Microscopy.

Olfactory bulb slices were blocked with 10% normal horse serum for 1 h. Antibodies were diluted in phosphate buffered saline containing 0.3% Triton X-100 and 0.04% NaN3. The entire procedure was conducted at room temperature.

Antibody Manufacturer Working
solution
Incubation
Anti-ETA Alomone, Jerusalem, ISR 1:500 18-20 h in rocking agitation
Anti-TH Sigma, Missouri, USA 1:10000
Anti-Rabbit-Alexa488 Jackson, Pensilvania,
USA
1:250 4 h in rocking agitation and light protected
Anti-Mouse-Alexa594 Jackson, Pensilvania,
USA
1:250
DAPI Sigma, Missouri, USA 1:20000 Last 5 min of secondary
antibody incubation
Anti-ETA Alomone, Jerusalem, ISR 1:500 18-20 h in rocking agitation
Anti-TH Sigma, Missouri, USA 1:10000
Anti-Rabbit-Alexa488 Jackson, Pensilvania,
USA
1:250 4 h in rocking agitation and light protected
Anti-Mouse-Alexa594 Jackson, Pensilvania,
USA
1:250
DAPI Sigma, Missouri, USA 1:20000 Last 5 min of secondary
antibody incubation