Table 1: Antibodies Used in Fluorescence Microscopy.
Antibody | Manufacturer | Working solution |
Incubation |
---|---|---|---|
Anti-ETA | Alomone, Jerusalem, ISR | 1:500 | 18-20 h in rocking agitation |
Anti-TH | Sigma, Missouri, USA | 1:10000 | |
Anti-Rabbit-Alexa488 | Jackson, Pensilvania, USA |
1:250 | 4 h in rocking agitation and light protected |
Anti-Mouse-Alexa594 | Jackson, Pensilvania, USA |
1:250 | |
DAPI | Sigma, Missouri, USA | 1:20000 | Last 5 min of secondary antibody incubation |
Anti-ETA | Alomone, Jerusalem, ISR | 1:500 | 18-20 h in rocking agitation |
Anti-TH | Sigma, Missouri, USA | 1:10000 | |
Anti-Rabbit-Alexa488 | Jackson, Pensilvania, USA |
1:250 | 4 h in rocking agitation and light protected |
Anti-Mouse-Alexa594 | Jackson, Pensilvania, USA |
1:250 | |
DAPI | Sigma, Missouri, USA | 1:20000 | Last 5 min of secondary antibody incubation |