Table 1: Antibodies Used in Fluorescence Microscopy.
Olfactory bulb slices were blocked with 10% normal horse serum for 1 h. Antibodies were diluted in phosphate buffered saline containing 0.3% Triton X-100 and 0.04% NaN3. The entire procedure was conducted at room temperature.
| Antibody | Manufacturer | Working solution |
Incubation |
|---|---|---|---|
| Anti-ETA | Alomone, Jerusalem, ISR | 1:500 | 18-20 h in rocking agitation |
| Anti-TH | Sigma, Missouri, USA | 1:10000 | |
| Anti-Rabbit-Alexa488 | Jackson, Pensilvania, USA |
1:250 | 4 h in rocking agitation and light protected |
| Anti-Mouse-Alexa594 | Jackson, Pensilvania, USA |
1:250 | |
| DAPI | Sigma, Missouri, USA | 1:20000 | Last 5 min of secondary antibody incubation |
| Anti-ETA | Alomone, Jerusalem, ISR | 1:500 | 18-20 h in rocking agitation |
| Anti-TH | Sigma, Missouri, USA | 1:10000 | |
| Anti-Rabbit-Alexa488 | Jackson, Pensilvania, USA |
1:250 | 4 h in rocking agitation and light protected |
| Anti-Mouse-Alexa594 | Jackson, Pensilvania, USA |
1:250 | |
| DAPI | Sigma, Missouri, USA | 1:20000 | Last 5 min of secondary antibody incubation |