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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Aug 6;1865(11):165527. doi: 10.1016/j.bbadis.2019.08.003

Table 2: Antibodies Used in Western Blot.

All membranes were blocked with 5% non-fat milk in TBS-T for 1 h at room temperature. Antibodies were diluted in 1% non-fat milk in TBS-T.

Antibodies Working
solution		 Incubation
Primary antibodies Anti TH 1:2000 18-20 h at 4°C
Anti β-Actin 1:2000
Anti TH-PSerl9 1:2000
Anti TH-PSer31 1:750
Anti TH-PSer40 1:2000
Anti ET-A 1:400
Anti ET-B 1:400
Secondary antibodies Anti-mouse (TH preincubation) 1:4000 90 min at room temperature with rocking agitation
Anti-rabbit (β-actin preincubation) 1:4000
Anti-rabbit (TH-PSerl9 preincubation) 1:5000
Anti-rabbit (TH-PSer31 preincubation) 1:500
Anti-rabbit (TH-PSer40 preincubation) 1:5000
Anti-rabbit (ET-A preincubation) 1:2000
Anti-rabbit (ET-B preincubation) 1:2000