Table 1. Primers and probes for the RT-qPCR.
Gene | Oligonucleotide | Sequence |
---|---|---|
E gene | E_ Sarbeco _F | ACAGGTACGTTAATAGTTAATAGCGT |
E_Sarbeco_P1 | FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ | |
E_Sarbeco_R | ATATTGCAGCAGTACGCACACA | |
RdRP gene | RdRp_SARSr-F | GTGARATGGTCATGTGTGGCGG |
RdRp_SARSr-P2 | FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ | |
RdRp_SARSr-R | CARATGTTAAASACACTATTAGCATA |
FAM: 6-carboxyfluorescein; BBQ: blackberry quencher.