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. 2020 Jul 20;14(7):e0008479. doi: 10.1371/journal.pntd.0008479

Fig 2. siRNA-mediated silencing of Aae Sh results in neural defects in A. aegypti adults.

Fig 2

The high levels of Aae Sh expression detected throughout the A. aegypti adult female brain (a control siRNA-treated brain is shown in A1) were significantly reduced in adults injected with Sh.463 siRNA (A2). Adult brains were labeled with mAbnc82 (white in B1, B2; red in C1, C2), which labels synaptic active zones, and the nuclear stain TO-PRO (blue in C1, C2). nc82 levels were significantly reduced (B3) in the synaptic neuropil of adult females injected with Sh.463 siRNA (B2, C2; compare to white staining of control siRNA-injected brain in B1/red staining in C1). In A3, B3, and C3, data are represented as average mean gray values, and error bars denote SEM; *** = P<0.001 when compared with control siRNA-injected mosquitoes. Representative adult brains are oriented dorsal upward in this figure. AL: antennal lobe; OL: optic lobe; SOG: sub-esophageal ganglion; SuEG: supra-esophageal ganglion. Scale Bar = 100 μm.