Table 2. Seroconversion of sentinel birds and birds infected with A/Gallus gallus/Belgium/3497_0001/2019 (H3N1) virus.
| Bird | 9 dpi |
14 dpi |
21 dpi |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ELISA-NP† |
HI‡ |
ELISA-NP† |
HI‡ |
ELISA-NP† |
HI‡ |
||||||||||
|
|
S/N, % |
IR |
log 2 |
IR |
|
S/N, % |
IR |
log 2 |
IR |
|
S/N, % |
IR |
log 2 |
IR |
|
| Infected 1 | 4.3 | + | 6 | + | 4.8 | + | 10 | + | 5.5 | + | 8 | + | |||
| Infected 2 | 5.0 | + | 6 | + | 5.6 | + | 9 | + | 4.7 | + | 9 | + | |||
| Infected 3 | 5.8 | + | 6 | + | 4.8 | + | 9 | + | 4.3 | + | 9 | + | |||
| Infected 4 | 10.2 | + | 3 | – | 9.0 | + | 3 | – | 12.6 | + | 3 | – | |||
| Infected 5§ | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | ||||
| Infected 6§ | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | ||||
| Average (SD) |
6.3 ±2.6 |
5.3 ±1.5 |
|
6.0 ±2.0 |
7.8 ±3.2 |
|
6.8 ±3.9 |
7.3 ±2.9 |
|||||||
| Sentinel 1 | 17.6 | + | 2 | – | 5.3 | + | 7 | + | 4.8 | + | 9 | + | |||
| Sentinel 2 | 5.9 | + | 2 | – | 4.9 | + | 7 | + | 4.8 | + | 7 | + | |||
| Sentinel 3 | 7.7 | + | 2 | – | 4.9 | + | 6 | + | 4.6 | + | 8 | + | |||
| Sentinel 4§ | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | ||||
| Sentinel 5 | 5.7 | + | 3 | – | 4.7 | + | 8 | + | 4.8 | + | 9 | + | |||
| Sentinel 6 | 5.6 | + | 2 | – | 4.4 | + | 7 | + | 5.5 | + | 8 | + | |||
| Average (SD) | 8.5 ±5.1 |
2.2 ±0.4 |
4.8 ±0.3 |
7.0 ±0.7 |
4.9 ±0.4 |
8.2 ± 0.8 |
|||||||||
*dpi, days postinfection; ELISA-NP, nucleoprotein competition ELISA; HI, hemagglutination inhibition; IR, immune response; NA, not applicable because of bird death; S/N, signal-to-noise ratio; +, positive immune response; –, negative immune response. †(IDVet, https://www.id-vet.com/). ELISA data are presented as the percentage of competition detected, the S/N. ‡HI test with the homologous H3N1 antigen. HI data are presented as log2 of the HI titer.