Table 1. Timeline of heart transplant patient with chronic Chagas disease from initial diagnosis to last follow-up and death*.

Date	Infection	Observation, treatment, outcome
2015 Aug	Cytomegalovirus, detected by serology	Diagnosed only by positive IgG serology, no active infection (no positive IgM serology). No treatment.
2015 Aug	Toxoplasmosis, detected by serology	Diagnosed only by positive IgG serology, no active infection (no positive IgM serology). No treatment.
2015 Nov		Heart transplantation on Nov. 28. Patient started with immunosuppressive therapy (tacrolimus, azathioprine, prednisone) until the end of follow-up.
2016 Jan	Chagas disease reactivation, detection by qPCR	Pretreatment sample collected on Jan 28. Patient started BZN treatment (2.5 mg/kg, twice a day, 60 d) on Feb 3.
2016 Mar	Bronchopulmonary aspergillosis, detected by serology and CT	BZN course interrupted on Mar 21. Completed 80% of the prescribed treatment.
2016 Mar	Bronchopulmonary aspergillosis	Aspergillosis treatment started on Mar 22. Initially with voriconazole and amphotericin B liposomal. Treatment was changed to posaconazole until the end of the follow-up.†
2016 Apr	Chagas disease reactivation, detected by qPCR	On Apr 14, patient started second round of BZN treatment until May 5, completing 100% of the prescribed treatment.
2016 May		Posttreatment sample collected on May 11.
2016 Aug		Late organ rejection. Patient died.

*BZN, benznidazole; CT, computed tomographic scan; qPCR, quantitative PCR.
†Parasite clearance could be related to the prolonged used of posaconazole, as previously reported (6), and/or the combined use of posaconazole and benznidazole because a second round of the latter was started in April 2016.