Table 2. Trypanosoma cruzi and human proteins identified in plasma-derived EVs from a heart transplant patient with chronic Chagas disease before benznidazole chemotherapy but absent after treatment and in healthy donors.
| Protein name† | UniProt accession no. | Unique peptides |
|||
|---|---|---|---|---|---|
| ChD Pre-BZN‡ | ChD Post-BZN | Healthy 1 | Healthy 2 | ||
| T. cruzi | |||||
| Pyruvate. phosphate dikinase OS = Trypanosoma cruzi
marinkellei GN = MOQ_000480 PE = 3 SV = 1 |
K2MVM1_TRYCR |
2 (0.96) |
0 |
0 |
0 |
| Homo sapiens | |||||
| Collagen α-1(VI) chain OS = Homo sapiens GN = COL6A1 PE = 1 SV = 3 | CO6A1_HUMAN | 3 (1.44) | 0 | 0 | 0 |
| Group of Angiopoietin-related protein 6 OS = Homo sapiens GN = ANGPTL6 PE = 1 SV = 1+1 | ANGL6_HUMAN (+1) | 3 (1.44) | 0 | 0 | 0 |
| sp|PPIA_HUMAN| | sp|PPIA_HUMAN| | 3 (1.44) | 0 | 0 | 0 |
| Mannan binding lectin serine protease 2 OS = Homo sapiens GN = MASP2 PE = 1 SV = 4 | MASP2_HUMAN | 2 (1.92) | 0 | 0 | 0 |
| Myosin regulatory light chain 12B OS = Homo sapiens GN = MYL12B PE = 1 SV = 2 | ML12B_HUMAN | 2 (1.92) | 0 | 0 | 0 |
| Collagen α-2(VI) chain OS = Homo sapiens GN = COL6A2 PE = 1 SV = 4 | CO6A2_HUMAN | 2 (1.44) | 0 | 0 | 0 |
| Collectin subfamily member 10 (C-type lectin). isoform CRA_a OS = Homo sapiens GN = COLEC10 PE = 4 SV = 1 | tr|A0A024R9J3|A0A024R9J3_HUMAN | 2 (1.44) | 0 | 0 | 0 |
| Group of Coagulation factor XIII A chain OS = Homo sapiens GN = F13A1 PE = 1 SV = 4+2 | F13A_HUMAN (+2) | 2 (1.44) | 0 | 0 | 0 |
| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein. eta polypeptide. isoform CRA_b OS = Homo sapiens GN = YWHAH PE = 3 SV = 1 | tr|A0A024R1K7|A0A024R1K7_HUMAN | 2 (1.44) | 0 | 0 | 0 |
| Fibrinogen-like protein 1 OS = Homo sapiens GN = FGL1 PE = 1 SV = 3 | FGL1_HUMAN | 2 (0.96) | 0 | 0 | 0 |
| Group of L-lactate dehydrogenase A chain OS = Homo sapiens GN = LDHA PE = 1 SV = 2+1 | LDHA_HUMAN (+1) | 2 (0.96) | 0 | 0 | 0 |
| Group of Laminin subunit α-2 OS = Homo sapiens GN = LAMA2 PE = 1 SV = 1+1 | A0A087WX80_HUMAN (+1) | 2 (0.96) | 0 | 0 | 0 |
| Group of MHC class I antigen (Fragment) OS = Homo sapiens GN = HLA-A PE = 3 SV = 1+3 | tr|D2KZ27|D2KZ27_ HUMAN (+3) | 2 (0.96) | 0 | 0 | 0 |
| Group of Serum amyloid A protein OS = Homo sapiens GN = SAA1 PE = 1 SV = 1+2 | E9PQD6_HUMAN (+2) | 2 (0.96) | 0 | 0 | 0 |
| Group of Transforming growth factor β-induced 68kDa isoform 2 (Fragment) OS = Homo sapiens GN = TGFBI PE = 2 SV = 1+1 | tr|A0A0S2Z4K6|A0A0S2Z4K6_HUMAN (+1) | 2 (0.96) | 0 | 0 | 0 |
| Heparan sulfate proteoglycan 2 (Perlecan). isoform CRA_b OS = Homo sapiens GN = HSPG2 PE = 4 SV = 1 | tr|A0A024RAB6|A0A024RAB6_HUMAN | 2 (0.96) | 0 | 0 | 0 |
| Neurogenic locus notch homologue protein 3 OS = Homo sapiens GN = NOTCH3 PE = 1 SV = 2 | NOTC3_HUMAN | 2 (0.96) | 0 | 0 | 0 |
| V1–16 protein (Fragment) OS = Homo sapiens GN = V1–16 PE = 4 SV = 1 | tr|Q5NV81|Q5NV81_ HUMAN | 2 (2.88) | 0 | 0 | 0 |
| Rheumatoid factor RF-ET6 (Fragment) OS = Homo sapiens PE = 2 SV = 1 | tr|A2J1N5|A2J1N5_ HUMAN | 2 (5.29) | 0 | 0 | 0 |
*BZN, benznidazole; ChD, Chagas disease. †All proteins were identified by at least 2 unique peptides. A unique peptide is defined as a peptide found only in >1 protein members of the same protein family of a certain proteome. ‡Normalized total spectrum count values are indicated in parenthesis. Complete mass spectrometry proteomic data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRoteomics IDEntifications Database (PRIDE, https://www.ebi.ac.uk/pride/) partner repository, with the dataset identifier PXD014668 and 10.6019/PXD014668.