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. 2020 May 14;79(8):1111–1120. doi: 10.1136/annrheumdis-2019-216911

Figure 2.

Figure 2

Global knockout and conditional knockout of FBXO6 in chondrocytes accelerated post-injury-induced cartilage degeneration. (A) Immunofluorescence of FBXO6 in global FBXO6 -/- mice and wide-type (WT) mice. (B) Safranin O-Fast Green staining of the knee joint at 8 weeks after sham and surgical induction of two genotypes of mice. (C) FBXO6-positive cell quantification and OARSI histological scores were compared between global FBXO6 -/- mice and WT mice. (D) Immunofluorescence for detection of FBXO6 in Col2a1-CreERT2;FBXO6f/f and WT mice 8 weeks after tamoxifen injection. (E) Safranin O-Fast Green staining of the knee joint at 8 weeks after sham and surgical induction of two genotypes of mice. (F) OARSI (Osteoarthritis Research Society International) histological scores (upper) and osteophyte volume qualification 8 weeks after sham or ACLT surgery (lower) were compared between two genotypes of mice. (G) Immunofluorescence for Aggrecan and Col-X in tibial cartilage at 8 weeks after sham or ACLT surgery. (Scale bars: 100 µm. Data are expressed as mean±SD. *<0.05; ***p<0.001.)