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. 2020 Jul 29;40(31):6018–6034. doi: 10.1523/JNEUROSCI.2674-19.2020

Table 2.

Primers used to amplify and clone tph-1p, ops-1p, gcy-17p, gcy-27p and flp-34 cDNA constructs in the pSM vectora

Plasmid PCR fragment Forward primer (5′-3′) Reverse primer (5′-3′)
pSM-flp-34 cDNA flp-34 cDNA ttggctagcgtcgacggtaccATGCAGTTCCAATTTTTGATG gtaggatgagacagcggtaccTTATTTACGGCCAAATCG
pSM-tph-1p::flp-34 cDNA tph-1p tcactcattaggcaccccagATATGGCTGCAATACTATTTTTC gcttggcgtaatcatggtcaCTAAAAACCAAAGAAAATCATGAAATG
pSM-ops-1p::flp-34 cDNA ops-1p tcactcattaggcaccccagGGAGATGAGCCAATATTTTTAAAAG gcttggcgtaatcatggtcaATTTATTTACAGGTAGCTTTTAGAG
pSM-gcy-17p::flp-34 cDNA gcy-17p tcactcattaggcaccccagCAGTTGAACATCTCCCTG gcttggcgtaatcatggtcaTAAAAGAAGCTTGCCCAATG
pSM-gcy-27p::flp-34 cDNA gcy-27p tcactcattaggcaccccagGCATTGTGTCAATTGCTC gcttggcgtaatcatggtcaTTGGTAGAAAATAAATAAAATGAAAGTTG

aUppercase nucleotides indicate the gene-specific sequence of the primer; lowercase nucleotides indicate the pSM vector overlapping sequence of the primer.