Table 2.
Plasmid | PCR fragment | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|---|
pSM-flp-34 cDNA | flp-34 cDNA | ttggctagcgtcgacggtaccATGCAGTTCCAATTTTTGATG | gtaggatgagacagcggtaccTTATTTACGGCCAAATCG |
pSM-tph-1p::flp-34 cDNA | tph-1p | tcactcattaggcaccccagATATGGCTGCAATACTATTTTTC | gcttggcgtaatcatggtcaCTAAAAACCAAAGAAAATCATGAAATG |
pSM-ops-1p::flp-34 cDNA | ops-1p | tcactcattaggcaccccagGGAGATGAGCCAATATTTTTAAAAG | gcttggcgtaatcatggtcaATTTATTTACAGGTAGCTTTTAGAG |
pSM-gcy-17p::flp-34 cDNA | gcy-17p | tcactcattaggcaccccagCAGTTGAACATCTCCCTG | gcttggcgtaatcatggtcaTAAAAGAAGCTTGCCCAATG |
pSM-gcy-27p::flp-34 cDNA | gcy-27p | tcactcattaggcaccccagGCATTGTGTCAATTGCTC | gcttggcgtaatcatggtcaTTGGTAGAAAATAAATAAAATGAAAGTTG |
aUppercase nucleotides indicate the gene-specific sequence of the primer; lowercase nucleotides indicate the pSM vector overlapping sequence of the primer.