Table 3.
gRNA sequences used to target flp-34 and dyp-10, and the respective repair template sequencesa
| Gene | gRNA/template | Sequence |
|---|---|---|
| flp-34 | gRNA 1 | CAGCGTACTATCCCTGCCACTGG |
| flp-34 | gRNA 2 | GATAACGTCCTTCCGAGTAGAGG |
| flp-34 | Repair template | TAGCTGTCGCTCCCTCCGAGGGTCGATACC |
| dpy-10 | gRNA | GCTACCATAGGCACCACGAG |
| dpy-10 | Repair template | CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAACCGTACCGCATGCGGTGCCTATGGTAGCGGAGCTTCACATGGCTTCAGACCAACAGCCTAT |
aThe dpy-10 gRNA and repair template have been previously reported (Arribere et al., 2014). Nucleotides in bold indicate a stop codon.