Table 3.
Primers used for real-time quantitative polymerase chain reaction
| Gene | Primer sequence, 5′→3′ 1 | Product size, bp | GenBank accession | Annealing Temperature, °C |
|---|---|---|---|---|
| Claudin-3 | F: TTGCATCCGAGACCAGTCC | 85 | NM_001160075 | 60 |
| R: AGCTGGGGAGGGTGACA | ||||
| Claudin-4 | F: CAACTGCGTGGATGATGAGA | 140 | NM_001161637 | 60 |
| R: CCAGGGGATTGTAGAAGTCG | ||||
| Occludin | F: TCGTCCAACGGGAAAGTGAA | 95 | NM_001163647 | 55 |
| R: ATCAGTGGAAGTTCCTGAACCA | ||||
| zonula occuludens-1 (ZO-1) | F: CTCTTGGCTTGCTATTCG | 197 | XM_003353439 | 55 |
| R: AGTCTTCCCTGCTCTTGC | ||||
| ribosomal protein L19 (RPL19) | F: AACTCCCGTCAGCAGATCC | 147 | AF_435591 | 55 |
| R: AGTACCCTTCCGCTTACCG |
1F, forward primer; R, reverse primer.