FIG 6.

Western immunoblot detection of tsr-encoded potential small peptides. (A) Western blot analysis, performed using an anti-his antibody, to detect His-tagged, tsr-encoded peptides/proteins. Protein samples from the cell envelope, cytoplasm, and secreted fraction were analyzed. (B) Table of predicted sizes of potential tsr-encoded small peptides. (C) Predicted membrane topology of peptides encoded by tsr21C, tsr22, and trs37. All three peptides are predicted to contain transmembrane regions. Membrane topology was predicted using SACS MEMSAT2 Transmembrane Prediction (18).