TABLE 5.
Strains and plasmids
| Strain or plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| S. aureus AH1263 | USA300 LAC isolate cured of plasmid LAC-p03 | 35 |
| S. aureus RN4220 | Restriction-deficient transformation recipient | 36 |
| S. aureus RKC885 | AH1263 pTsr17-his | This study |
| S. aureus RKC886 | AH1263 pTsr18-his | This study |
| S. aureus RKC887 | AH1263 pTsr21B-his | This study |
| S. aureus RKC888 | AH1263 pTsr21C-his | This study |
| S. aureus RKC889 | AH1263 pTsr22-his | This study |
| S. aureus RKC681 | AH1263 pTsr37-his | This study |
| S. aureus RKC895 | AH1263 pTsr9-his | This study |
| E. coli DH5α | Cloning strain | Invitrogen |
| Plasmids | ||
| pMK4 | Gram-positive shuttle vector (CMr)a | 37 |
| pRKC679 | pMK4_Tsr37_6x-his (vector overexpressing His-tagged tsr37 from its native promoter) | This study |
| pRKC885 | pMK4_Tsr17_6x-his (vector overexpressing His-tagged tsr17 from its native promoter) | This study |
| pRKC886 | pMK4_Tsr18_6x-his (vector overexpressing His-tagged tsr18 from its native promoter) | This study |
| pRKC887 | pMK4_Tsr21B_6x-his (vector overexpressing His-tagged tsr21B from its native promoter) | This study |
| pRKC888 | pMK4_Tsr21C_6x-his (vector overexpressing His-tagged tsr21C from its native promoter) | This study |
| pRKC889 | pMK4_Tsr22_6x-his (vector overexpressing His-tagged tsr22 from its native promoter) | This study |
| pRKC892 | pMK4_Tsr9_6x-his (vector overexpressing His-tagged tsr9 from its native promoter) | This study |
a
CMr, chloramphenicol resistance.