TABLE 1.
lytA qPCR result | Microarray result | NPb samples (n = 250) | OPc samples (n = 250) |
---|---|---|---|
Positive (CT, <35) | Positive | 13 | 7 |
Negative | 0 | 2 | |
No growthd | 1 | 0 | |
Equivocal (CT, 35–40) | Positive | 7 | 3 |
Negative | 0 | 30 | |
No growthe | 12 | 1 | |
Negative (no CT) | 217 | 207 |
Samples were screened by lytA quantitative real-time PCR, and positive and equivocal samples were further tested by culture on selective agar and DNA microarray.
NP, nasopharyngeal.
OP, oropharyngeal.
One NP sample was nonculturable and therefore not tested by microarray.
Equivocal samples with no growth were considered negative for pneumococci (54).