Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | BIN1 | NCBI | Gene ID 274 | |
| Antibody | Anti-NeuN Rabbit polyclonal |
abcam | Cat# ab104225; RRID:AB_10711153 | ICC (1:500), Lot #GR3321966-1 |
| Antibody | Anti-GAD67 Mouse monoclonal |
Millipore Sigma | Cat# MAB5406; RRID:AB_2278725 | ICC (1:500), Lot #3015328 |
| Antibody | Anti-BIN1 Rabbit polyclonal |
Santa Cruz | Cat# sc-30099; RRID:AB_2243399 | ICC/PLA/IP (1:500), Lot #K1605; H-100 |
| Antibody | Anti-LVGCC-β1 Mouse monoclonal |
abcam | Cat# ab85020; RRID:AB_1861569 | ICC/PLA/IP (1:1000), Lot #413-8RR-52 |
| Antibody | Anti-Tau Rabbit polyclonal |
DAKO | Cat# A0024; RRID:AB_10013724 | ICC/IP (1:1000), Lot #20031827 |
| Antibody | Anti-BIN1 Mouse monoclonal |
Santa Cruz | Cat# sc-13575; RRID:AB_626753 | ICC/IP (1:1000), Lot #L3014; 99D |
| Cell line (Rattus norvegicus) |
Primary neuron | Charles River | Fresh from E19 albino Sprague Dawley rats | |
| Genetic reagent | AAV-BIN1-mKate2 | UPenn Vector Core | AAV2 | |
| Genetic reagent | AAV-mKate2 | UPenn Vector Core | AAV2 | |
| Sequence-based reagent | Tau ASO | PMID:23904623
IDT |
5-ATCACTGATTTTGAAGTCCC-3 | |
| Sequence-based reagent | Scrambled ASO | PMID:23904623
IDT |
5-CCTTCCCTGAAGGTTCCTCC-3 | |
| Commercial assay, kit | Duolink PLA kit | Millipore Sigma | Cat#s DUO92014; DUO92002; DUO92004 | |
| Transfected construct | GCaMP6f | Addgene | RRID:Addgene_40755 | |
| Transfected construct | mKate2 | PMID:25156556
Evrogen |
Cat# FP184 | Actin was removed from the construct obtained |
| Transfected construct (Homo sapiens) | BIN1 | Horizon Discovery ORFeome Collaboration Clones | OHS5894-202501160 | Isoform 1 |
| Cell line (Cricetulus griseus) |
CHO-K1 | Millipore Sigma | Cat# 85051005-1VL | Chinese Hamster Ovary cell line |
| Transfected construct | mKate2-Tau-mKate2 | PMID:25156556 | ||
| Transfected construct | Fyn-SH3-CBG | PMID:25156556 | BIN1-SH3 or LVGCC-β1-SH3 was cloned in replacing Fyn-SH3 | |
| Transfected construct (Homo sapiens) | BIN1-SH3 | IDT | AAC28646.1 | Codon optimized |
| Transfected construct (Homo sapiens) | LVGCC-β1-SH3 | IDT | AAA35632.1 | Codon optimized |