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. 2020 Jul 28;11(30):2906–2918. doi: 10.18632/oncotarget.27658

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Copyright: Oshima et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Immunohistochemical studies were performed for proteins encoded by seven biomarker candidate genes, ZDHHC14, TIMP1, CLDN18.2, EGFR, RRM1, MUC2 and DSG2 using formalin-fixed, paraffin-embedded biopsy specimens obtained from 24 patients. Immunohistochemical evaluation was performed based on the positive immunostained tumor cells with maximum intensity and the percentage of positive immunostained tumor cells. Representative images of high and low immunostaining of each biomarker candidate are shown.