Skip to main content
. 2020 Jul 28;11(30):2889–2905. doi: 10.18632/oncotarget.27587

Figure 4. Effect of exogenous HA ± 1,25D3 on growth and morphology of mammary tumor cells.

Figure 4

(A) KO240, WT145 and KOhVDR cells were treated with 500 mg/mL high molecular weight (HMW) or low molecular weight (LMW) HA in the presence or absence of 100 nM 1,25D3 for 96 hours. Adherent cell growth was measured by crystal violet absorbance. Bars represent mean ± standard error of 2 independent experiments analyzed in quadruplicate. * p < 0.05, control vs individual treatments assessed by one-way ANOVA. (B) Phase contrast images of WT145 cells 96 hours after treatment with vehicle, 500 mg/mL HMW HA, 100 nM 1,25D3 or the combination of 1,25D3 and HA.