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. 2020 Jul 28;11(30):2889–2905. doi: 10.18632/oncotarget.27587

Figure 6. Changes in VDR and HA pathway during EMT.

Figure 6

(A–C) RNA was isolated from immortalized human mammary epithelial (HMLE) cells stably expressing GFP (control), TGFβ, SNAIL or TWIST. Expression of VDR (A), HAS2 (B) and HAS2AS1(C) were evaluated by RT-qPCR, normalized to 18S expression and expressed relative to GFP control cell line. Bars represent mean ± standard deviation of at 3 independent samples analyzed in triplicate. * p < 0.05 vs expression in HMLE cells as determined by one-way ANOVA and Dunnett’s post hoc test. (D) Secreted HA was evaluated in conditioned media of HMLE cells stably expressing GFP, TGFβ, SNAIL or TWIST by ELISA. * p < 0.05, 1,25D3 treated vs control for each cell line as evaluated by Student’s t test.