Figure 8. Effect of H-RAS^V12 transformation on HAS gene expression in immortalized human mammary epithelial cells.

RNA was isolated from control human mammary epithelial (HMLE) cells and those expressing oncogenic H-RAS^V12 (HMLE-RAS) 24 hours after treatment with 100 nM 1,25D3 or vehicle. HAS2, HAS1, and HAS3 expression was evaluated by RT-qPCR, normalized to 18S mRNA and expressed relative to untreated HMLE cells. Bars represent mean ± standard deviation of 3 independent samples analyzed in quadruplicate. ^* p < 0.05, 1,25D3 treated vs control in each cell line as evaluated by Student’s t test.