Fig. 3.

GFI axonal projection overgrowth begins early in synaptogenesis. a The giant fiber interneuron (GFI) axonal bend visualized with 91H05-Gal4 driven UAS-mcd8::gfp (green) during staged development in background controls (w¹¹¹⁸, left) and dfmr1 null mutants (dfmr1^50M, right). The three time points are during (1) initial GFI synaptogenesis at 34–50 h after puparium formation (APF, top), (2) GFI synapse maturation at 73–78 h APF (center), and (3) GFI synapse stabilization at 75–90 h APF (bottom). Arrows indicate representative GFI axonal bend projections. Scale bar, 10 μm. b Quantification of the axonal projections at all 3 time points for both genotypes. Each gray dot represents the projection number for an axon bend in one animal. The black dot represents the mean and red bars represent the standard error of the mean. Sample sizes: 34–50 h: w¹¹¹⁸, n = 12; dfmr1^50M, n = 13. 73–78 h: w¹¹¹⁸, n = 10; dfmr1^50M, n = 10. 75–90 h: w¹¹¹⁸, n = 12; dfmr1^50M, n = 12