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. 2020 Jul 29;11(7):594. doi: 10.1038/s41419-020-02784-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The expression level of circATRNL1 in 60 ovary endometriosis tissue samples was examined by using qRT-PCR. bcircATRNL1 was overexpressed in Ishikawa cells by transfecting with lentivirus-mediated vector pHBLV-ATRNL1. NC was used as a control. ccircATRNL1 was silenced in Ishikawa cells by transfecting with specific sh-ATRNL1. shRNA-NC was used as a control. d CCK-8 assays were conducted to determine the influence of circATRNL1 on cell proliferation. e Transwell migration assays showed that circATRNL1 regulated the migrative potential of Ishikawa cells. Photographs were taken at ×200 magnification. Scale bar represents 100 μm. f Transwell invasion assays showed that circATRNL1 regulated the invasive potential of Ishikawa cells. g Western blot analysis was utilized to analyze the impacts of circATRNL1 overexpression or knockdown on EMT progress in Ishikawa cells. h Immunohistochemistry of EMT markers in 60 EuEM and EcEM tissues. Original magnification: ×400. Scale bars represent 50 μm. i Western blot analysis of EMT markers in 60 EuEM and EcEM tissues. Assays were performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 represent statistical difference.