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. 2020 Jul 29;11(7):594. doi: 10.1038/s41419-020-02784-4

Fig. 4. YAP1 is a common target mRNA of miR-141-3p and miR-200a-3p.

Fig. 4

a The downstream target mRNAs of miR-141-3p/miR-200a-3p were predicted by three bioinformatics tools (Targetscan, MiRDB, and mirTarbase). b The binding sites between 3′UTR of YAP1 and miR-141-3p/miR-200a-3p were predicted by using bioinformatics analysis. c Dual-luciferase reporter assays were carried in HEK-293T cells which were co-transfected with miR-141-3p mimics or miR-200a-3p mimics and pmiR–pmiR-GLO-YAP1. d The expression levels of YAP1 mRNA were tested in ovary endometriosis tissues through using qRT-PCR. e, f Immunohistochemistry and western blot analysis were used to test YAP1 protein in ovary endometriosis tissues. Immunohistochemistry photographs were taken at ×400 magnification. Scale bars represent 50 μm. ****P < 0.0001 represents statistical difference.