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. 2020 Jul 29;11(7):594. doi: 10.1038/s41419-020-02784-4

Fig. 6. Function of circATRNL1–miR-141-3p/miR-200a-3p–YAP1 axis in endometriosis progression.

Fig. 6

a, b mRNA level and protein level of YAP1 were measured by using qRT-PCR and western blot in Ishikawa cells which were co-transfected with miR-141-3p/miR-200a-3p mimics and pHBLV-ATRNL1 or miR-141-3p/miR-200a-3p inhibitors and sh-ATRNL1 or YAP1-si and pHBLV-ATRNL1. c–e CCK-8 and transwell assays were separately performed to detect proliferation and motility ability of indicated Ishikawa cells. Photographs were taken at ×200 magnification. Scale bar represents 100 μm. f The effect of circATRNL1–miR-141-3p/miR-200a-3p-YAP1 axis on EMT protein markers in Ishikawa cells was evaluated through western blot analysis. g The mechanism diagram was generated to illustrate the mechanism of circATRNL1–miR-141-3p/miR-200a-3p–YAP1 axis in endometriosis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 represent statistical difference.