Table 1.
Primers and probes used for the qPCR assays to detect S. pneumoniae, H. influenzae, S. aureus, M. catarrhalis, K. pneumoniae and M. pneumoniae and Multiplex PCR to detect H. influenzae capsule gene
| Organism | Target gene | Primers and probes |
|---|---|---|
| S. pneumoniae | lytA | |
| Forward primer | lytA-CDC-f, 5’-ACGCAATCTAGCAGATGAAGCA-3’ | |
| Reverse primer | lytA-CDC-r, 5’-TCGTGCGTTTTAATTCCAGCT-3’ | |
| Probe | lytA-CDC-pr, 5’-FAM-GCCGAAAACGCTTGATACAG GGAG-3-BHQ1 | |
| H. influenzae | Hpd | |
| Forward primer | hpdF729, 5’-AGATTGGAAAGAAACACAAGAAAAAGA-3’ | |
| Reverse primer | hpdR819, 5’-CACCATCGGCATATTTAACCACT-3’ | |
| S. aureus | nuc | |
| Forward primer | nucF, 5′-GTTGCTTAGTGTTAACTTTAGTTGTA-3′ | |
| Reverse primer | nucR, 5′-AATGTCGCAGGTTCTTTATGTAATTT-3′ | |
| M. catarrhalis | copB | |
| Forward primer | copBF, 5’-CGTGTTGACCGTTTTGACTTT-3’ | |
| Reverse primer | copBR, 5’-TAGATTAGGTTACCGCTGACG-3’ | |
| K. pneumoniae | phoE | |
| Forward primer | phoE_F, 5’- TGCCCAGACCGATAACTTTA-3’ | |
| Reverse primer | phoE_R, 5’- CTGTTTCTTCGCTTCACGG-3’ | |
| M. pneumoniae | ||
| Forward primer | mpn141 | Mpn141_F, 5’-AAGCACGAGTGACGGAAACAC-3’ |
| Reverse primer | Mpn141_R, 5’-CACCACATCATTCCCCGTATTAG-3’ | |
| Probe | Mpn141_P, 5’-FAM-CTCCACCAACAACCTCGCGCCTA-3-TAMRA | |
| H. influenzae | bexA | |
| Forward primer | Hi-1, 5’-CGTTTGTATGATGTTGATCCAGAC-3’ | |
| Reverse primer | Hi-2, 5’-TGTCCATGTCTTCAAAATGATG-3’ | |