Table 2.

Quantitative Summary of CS Changes in Response to Thrombin-Induced Platelet Activation and Their Putative Contribution to an Expanded Platelet Surface Area

	Plasma Membrane Surface Area (μm2)	Closed Canalicular System Surface Area (μm2)	Open Canalicular System Surface Area (μm2)	Total Canalicular System Surface Area (μm2)	Putative CS Mobilized for PM Expansion (μm2)	CS Surface Area Needed for PM Expansion (μm2)
0 seconds Activated ($\overline{X}\pm {\sigma }_{\overline{X}}$)	19 ± 2.4	4.0 ± 0.52	3.9 ± 1.9	7.9 ± 2.0	N/A	N/A
90 seconds Activated ($\overline{X}\pm {\sigma }_{\overline{X}}$)	25 ± 2.4	2.5 ± 0.45	0.81 ± 0.28	3.3 ± 0.53	4.6 ± 2.0	6.0 ± 3.4
300 seconds Activated ($\overline{X}\pm {\sigma }_{\overline{X}}$)	29 ± 3.6 (P = 0.089, 0 sec vs. 300 sec)	1.5 ± 0.72 (P = 0.04, 0 sec vs. 300 sec)	0.67 ± 0.24 (P = 0.14, 0 sec vs. 300 sec)	2.2 ± 0.76 (P = 0.03, 0 sec vs. 300 sec)	5.6 ± 2.1	10 ± 4.3

Cell surface and CS parameters were quantified for 5 fully rendered platelets at each time point. The blood from 6 mice, mixed gender, was pooled for each time point preparation.