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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Neurochem Int. 2020 May 23;138:104770. doi: 10.1016/j.neuint.2020.104770

Fig. 7. A2aR agonist and A3 antagonist do not block actions of ADX88178.

Fig. 7.

BV2 microglia were pre-treated with ADX88178 and A2a receptor agonist CGS21680 or A3 receptor antagonist MRS1334 to determine if adenosine receptors are mGluR4 binding partners. (A) LPS stimulation resulted in a significant increase in nitrite release (####P<0.0001 vs. control). Pre-treatment with ADX88178 significantly reduced LPS-induced nitrite production concentration-dependently (****P<0.0001 vs. LPS) while pretreatment with CGS21680 (50μM) or MRS1334 (50μM) prior to LPS stimulation did not cause any changes in the NO levels. Pretreatment with neither CGS21680 nor MRS1334 prior to LPS+ADX88178 caused inhibition of ADX88178 anti-inflammatory action. (B) There was no observed cytotoxicity at any concentration of ADX88178, CGS21680, or MRS1334 alone or in combination with LPS. All values are expressed as mean ± S.E.M. for at least three independent experiments. Data were analyzed using one-way ANOVA with post hoc Tukey’s test. ####P<0.0001 in comparison with control; ****P<0.0001 comparison with LPS.