Table 1:
Primary antibodies used in histological assays
| Antigen (Host) | Immunogen | Manufacturer, Product #, and Research Resource Identifier (RRID) | Concentration |
|---|---|---|---|
| Calbindin (mouse, monoclonal) | Purified from chicken gut | Swant Cat# 300, RRID:AB_10000347 | 1:2000 |
| Calretinin (mouse, monoclonal) | Recombinant human | Swant Cat# 6B3, RRID:AB_10000320 | 1:500 |
| Ca2+/calmodulin-dependent protein kinase II (6G9 clone) (mouse, monoclonal) | Partially purified rat CaMKII, purified from hybridoma tissue culture supernatant | Enzo Life Sciences Cat# ADI-KAM-CA002, RRID:AB_10617228 | 1:2000 |
| Choline acetyltransferase (goat, polyclonal) | Human placental enzyme | Millipore Cat# AB144P, RRID:AB_2079751 | 1:200 |
| Dopamine- and cAMP-regulated neuronal phosphoprotein-32 (total protein; mouse, monoclonal) | BALB/c spleen cells immunized with purified DARPP-32 and fused with SP2/0 Ag14 meyloma cells | Courtesy of Drs. Paul Greengard and Angus Nairn, RRID:AB_2811192 | 1:500 |
| Doublecortin (goat, polyclonal) | Peptide mapping at the C-terminus (amino acids 385–402) of Doublecortin of human origin | Santa Cruz Biotechnology Cat# sc-8066, RRID:AB_2088494 | 1:100 |
| Discosoma sp. red fluorescent protein (DsRed, rabbit, polyclonal) | Variant of Discosoma sp. (DsRed-Express) | Takara Bio Cat# 632496, RRID:AB_10013483 | 1:1000 (1:500 in RNAScope/IHC assay) |
| Enhanced green fluorescent protein (chicken, polyclonal) | Isolated directly from the jellyfish (Aequorea victoria) | Thermo Fisher Scientific Cat# A10262, RRID:AB_2534023 | 1:250 |
| Glutamic acid decarboxylase-67 (1G10.2 clone, mouse, monoclonal) | Recombinant GAD67 protein | Millipore Cat# MAB5406, RRID:AB_2278725 | 1:500 |
| Glial fibrillary acidic protein (GA5 clone, mouse, monoclonal) | Purified GFAP from porcine spinal cord | Millipore Cat# MAB360, RRID:AB_11212597 | 1:300 |
| NeuN (mouse) | Purified cell nuclei from mouse brain | Millipore Cat# MAB377, RRID:AB_2298772 | 1:100 |
| Parvalbumin (mouse, monoclonal) | Frog muscle parvalbumin | Sigma-Aldrich Cat# P3088, RRID:AB_477329 | 1:200 |
| Somatostatin (rat, monoclonal) | Synthetic peptide corresponding to amino acids 1–14 of cyclic SST conjugated to bovine thyroglobulin using carbodiimide | Millipore Cat# MAB354, RRID:AB_2255365 | 1:200 |
| Tyrosine hydroxylase, clone TH-2 (mouse, monoclonal) | Rat tyrosine hydroxylase | Sigma-Aldrich Cat# T1299, RRID:AB_477560 | 1:500 |
| Vimentin, clone LN-6 (mouse, monoclonal) | Nuclei from adult human thymic biopsy specimens | Millipore, Cat# MAB1681, RRID:AB_11211549 | 1:250 |