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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Comp Neurol. 2020 Mar 29;528(14):2445–2470. doi: 10.1002/cne.24905

Table 2:

Secondary antibodies used in histological assays

Fluorescent Conjugate (Host, Target) All are α-IgG (H+L) unless noted Jackson ImmunoResearch (West Grove, PA) Product # Concentration
Cyanine Cy3 (donkey α-rabbit) 711-165-152 1:1000
Alexa Fluor 488 (bovine α-goat) 805-545-180 1:1000
Alexa Fluor 488 (donkey α-mouse) 715-545-150 1:1000
Alexa Fluor 488 (goat α-mouse, IgG + IgM H+L) 115-545-068 1:500 Vimentin only
Cyanine Cy2 (donkey α-rat) 712-225-150 1:1000
Alexa Fluor 488 (donkey α-chicken) 703-545-155 1:1000
Biotin (donkey α-mouse) 715-065-150 1:1000
Biotin (donkey α-rat) 712-065-150 1:1000
Horseradish peroxidase (donkey α-rabbit) 711-035-152 1:1000