Fig. 3.
APP is secreted into vesicles independent of ESCRT machinery. a GFP-tagged wild-type Vps4a and corresponding dominant negative construct (E228Q) were transfected into HEK293 cells expressing APPswe. Upper bands in CTFβ blots likely represent protein dimers due to a molecular weight of approximately 20 kDa and recognition by the same specific antibody. b Immunoblot analysis of EVs secreted from Vps4a WT and dominant negative transfected cells. Blots are representative images from three to four repeated independent experiments. Resultant quantitation of (c) vesicle APPswe and CTFβ, (d) Hrs, and (e) Tsg101 vesicle secretion, normalized to cellular levels. One-way ANOVA was used to determine statistical significance across samples from three independent experiments. *, p < 0.05