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. 2020 Jul 30;21:58. doi: 10.1186/s12860-020-00302-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Wild-type APP is similarly dependent upon Alix and Syntenin-1 for secretion. a Inducible shRNA knockdown cells were transfected with wild-type APP (APP^WT), equal mass loaded. b EVs were harvested from APP^WT transfected cells for immunoblot analysis, equal volume loaded. c Quantitation of APP^WT secreted into EVs from knockdown cells, normalized to cellular levels. d GFP-tagged wild-type Vps4a and corresponding dominant negative construct (Vps4a E228Q) were transfected into HEK293 cells expressing APP^WT. EVs were collected and analyzed by immunoblot analysis, equal volume loaded. e Quantitation of APP^WT secreted into EVs from wild-type Vps4a and dominant negative Vps4a transfected cells. Blots are representative images from three to five repeated independent experiments. T-test: *, p < 0.05; **, p < 0.01