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. 2020 Jul 30;21:58. doi: 10.1186/s12860-020-00302-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — APP endolysosomal localization is altered in the absence of Alix and Syntenin-1. a APP immunofluorescent staining was performed on control HEK293 cells expressing APP^swe or following induction of Alix (shAlix) or Syntenin-1 (shSyntenin-1) shRNA knockdown. To assess subcellular compartment localization of APP^swe in the absence of Alix and Syntenin, GFP-tagged APP^swe was introduced into cells. Cells were stained with (b) DHPE or (c) Lysotracker before live-cell imaging on a Zeiss LSM 880 confocal microscope. Images are representative across at least three independent experiments. Scale bar = 20 μm. Pearson’s Correlation Coefficient (PCC) calculated from ≥10 cells. One-way ANOVA: **, p < 0.01. *, p < 0.05