Table 2.
Phenotypes of overexpression and/or knockdown of specific isoform.
| Genotype | Phenotypes | Pupal lethality % |
|---|---|---|
| hth > Hth-C | Reduction or absence of eyes; deformed ocellar region; deformed or absence of palps; antenna with shorter a3 segment; some with ectopic antennal a2 segment in eye region; some with no or only one haltere; and disordered vibrissae | 78% (n = 49) |
| hth > Hth-A | Similar to hth > Hth-C. Headless (12%) or wingless (6%) | 87% (n = 54) |
| hth > miR-A | Normal head capsule and clypeus. No antenna-to-leg transformation | 100% (n = 154) |
| hth > miR-C | Normal head capsule and clypeus. No antenna-to-leg transformation | 100% (n = 125) |
| hth > miR-A + Hth-A | 58% fully rescued. Some with deformed eyes and palps. Some with shorter antennae | 6% (n = 135) |
| hth > miR-A + Hth-C | 66% fully rescued. Pharate adults have deformed palps; shorter antennae | 5% (n = 101) |
| hth > miR-C + Hth-A in hth1422-1/ + | Small or no eyes; disordered vibrissae; no or reduced ocelli; no or deformed palps; shorter antennal a3 segment. 31% pupae without head | 98% (n = 67) |
| hth > miR-C + Hth-C in hth1422.4/ + | Shorter antennae, deformed or no palps and disordered vibrissae | 58% (n = 33) |
The phenotypes of overexpressing isoforms Hth-A and Hth-C and specific knockdown by miR-A and miR-C are described. The phenotypes of coexpressing different miR with Hth-A or Hth-C are also described.