Fig. 3. RTP801 is increased in the synaptic fraction derived from the striatum of HdhQ⁷/Q¹¹¹ mice.

a, b Striatal homogenates (H) and synaptosomes (S) of six KI and six WT animals at 10-months of age were subjected to WB. Membranes were probed against RTP801, P-Akt (Ser473), P-S6 (Ser235/236), PSD-95, SV2A and total Akt was used as loading control. Graphs show the densitometric quantification of synaptosomal levels. c, d Graphs indicate the levels of RTP801, P-Akt (Ser473), and P-S6 (235/236) relative to synaptic markers c SV2A and d PSD-95 in the synaptosomes. The results are shown as mean ± SEM. Data were analyzed by Student’s t-test (*P < 0.05, **P < 0.01).