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. 2020 Jul 30;11(7):569. doi: 10.1038/s41419-020-02775-5

Fig. 6. Knockdown of striatal RTP801 in R6/1 mice prevents hyperphosphorylation of Akt-(Ser473) by decreasing Rictor levels.

Fig. 6

Striatal homogenates (H) and synaptosomes (S) of WT and R6/1 injected with AAV-shCtr (n = 6 WT and n = 6 R6/1) or AAV-shRTP801 (n = 6 WT and n = 7 R6/1) were subjected to WB. Membranes were probed against a P-mTOR (Ser2448), b P-S6 (Ser235/236), c P-Akt (Ser273), d Rictor, and e PHLPP1. Total mTOR, S6, Akt, and actin were used as loading controls. Graphs show the densitometric quantification. Values are shown as a mean ± SEM. Homogenates and synaptosomes data were analyzed with two-way ANOVA followed by Bonferroni’s multiple comparisons test for post hoc analyses (*P < 0.05, **P < 0.01, **P < 0.010 vs. WT AAV-shCtr; $P < 0.05 vs. WT AAV-shRTP801; #P < 0.05, ##P < 0.01 vs. R6/1 AAV-shCtr).