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. 2020 Jul 30;11:3810. doi: 10.1038/s41467-020-17665-9

Fig. 4. ORF6 antagonizes innate immune response via its C terminus.

Fig. 4

a Schematic diagram of SARS-CoV-2 ORF6 variants. ORF6-WT: wildtype, ORF6-M1: amino acids 49–52 were substituted with alanines, ORF6-M2: amino acids 53–55 were substituted with alanines; ORF6-M3: amino acids from 56 to 61 were substituted with alanines. b–e The effect of ORF6 mutants on IFN-β promoter activation. HEK293T cells were transfected with an IFN-β reporter plasmid, along with a control plasmid or plasmids expressing wildtype ORF6 or indicated ORF6 variants, together with plasmids expressing RIG-IN (b), MDA5 (c), MAVS (d), or IRF3-5D (e). At 24 h after transfection, luciferase activity was measured. Protein expression levels were detected by Western blot. All experiments were done at least twice, and one representative is shown. Error bars indicate SD of technical triplicates. *P < 0.05, **P < 0.01, and ***P < 0.001, ns not significant, two-tailed Student’s t-test. Source data are provided as a Source Data file.