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. 2020 Jul 30;11:3810. doi: 10.1038/s41467-020-17665-9

Fig. 5. ORF6 inhibits ISRE promoter activation.

Fig. 5

a The effects of SARS-CoV-2 proteins on ISRE-promoter activation. HEK293T cells were transfected with an ISRE reporter plasmid, along with a control plasmid or with plasmids expressing the indicated SARS-CoV-2 proteins. At 24 h post-transfection, cells were treated with 800 U/ml of human IFN-β for 12 h, and luciferase activity was measured. b, c The effects of SARS-CoV-2 ORF6 on ISRE and ISG56 promoter activity. HEK293T cells were transfected with am ISRE reporter plasmid (b) and an ISG56 reporter plasmid (c), along with a control plasmid or the increasing amounts of plasmids expressing SARS-CoV-2 ORF6. At 24 h post-transfection, cells were treated with 800 U/ml of human IFN-β for 12 h, and luciferase activity was measured. d, e Effect of SARS-CoV ORF6 and SARS-CoV-2 ORF6 on ISRE and ISG56 promoters. HEK293T cells were transfected with an ISRE reporter plasmid (d) or an ISG56 reporter plasmid (e), along with a control plasmid or plasmids expressing SARS-CoV ORF6 or SARS-CoV-2 ORF6. At 24 h post-transfection, cells were treated with 800 U/ml of human IFN-β for 12 h, and luciferase activity was measured. All experiments were done at least twice, and one representative is shown. Error bars indicate SD of technical triplicates. *P < 0.05, **P < 0.01, and ***P < 0.001, two-tailed Student’s t-test. Source data are provided as a Source Data file.