Table 4. Mutation of PrtS amino acid sequences in slow acidifying prtS+ strains.
| Slow acidifying prtS+ strains | Accession No. of prtS | Domain | Mutation type | Predicted amino acid change |
|---|---|---|---|---|
| ME-722 | LC514594 | H | misssense | Thr1140Ile, Leu1189Ser, Asp1191Glu, Ile1211Val, Ala1232Thr, |
| Glu1294Lys, Ala1342Thr, Tyr1380phe, Asn1424Gly, Val1475Ala | ||||
| W | misssense | Ile1533Lys, Glu1548Gly | ||
| AN | misssense | Lys1593Arg, Pro1598Leu, Ile1611Lys | ||
| ME-731 | LC514595 | PP | misssense | Leu38Val, Ala53Val, Gly59Ala |
| ME-751 | LC514596 | PP | misssense | Leu38Val, Ala53Val, Gly59Ala |
| PR | frameshift | Ala316Serfs*13 | ||
| ME-761 | LC514597 | PP | frameshift | Leu26Phefs*12 |
| ME-764 | LC514598 | SS | misssense | Val19Ile |
| A | frameshift | Thr650Asnfs*41 | ||
| ME-766 | LC514599 | A | misssense | Asn937Asp, Asp944Asn |
| A | nonsense | Trp958* |
Predicted PrtS amino acid sequences of 6 slow acidifying prtS+ strains (accession Nos. are listed in the table) were compared with those of the PrtS of S. thermophilus LMD-9 (accession No. ABJ66087) and 4F44 (accession No. ADB77872). Domain names are abbreviated as follows: PP: signal sequence; PR: catalytic domain; A: globular domain; H: helical domain; W: wall domain; AN: anchored domain [8].