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. 2020 Feb 19;39(3):89–99. doi: 10.12938/bmfh.2019-025

Table 1. Real-time PCR primers.

Target Sequences Amplicon (bp) Tm (℃)
β-actin Purchased from Sangon Biotech (Shanghai) Co., Ltd. Number: B661302 174 60
BDNF F: 5´- TGG AAC TCG CAA TGC CGA ACT AC -3´ 88 58
R: 5´- TCC TTA TGA ATC GCC AGC CAA TTC TC -3´
GABAAα1 F: 5´- AAA AGT CGG GGT CTC TCT GAC -3´ 138 58
R: 5´- CAG TCG GTC CAA AAT TCT TGT GA -3´
GABAb1 F: 5´- ACG TCA CCT CGG AAG GTT G -3´ 107 57
R: 5´- CAC AGG CAG GAA ATT GAT GGC -3´
5-HT1A F: 5´- TGG GCA ATC ACC GAC CCT AT -3´ 179 55.5
R: 5´- TAC CCG TGG TCC TTG CTG AT -3´