Table 1. Real-time PCR primers.
Target | Sequences | Amplicon (bp) | Tm (℃) |
---|---|---|---|
β-actin | Purchased from Sangon Biotech (Shanghai) Co., Ltd. Number: B661302 | 174 | 60 |
BDNF | F: 5´- TGG AAC TCG CAA TGC CGA ACT AC -3´ | 88 | 58 |
R: 5´- TCC TTA TGA ATC GCC AGC CAA TTC TC -3´ | |||
GABAAα1 | F: 5´- AAA AGT CGG GGT CTC TCT GAC -3´ | 138 | 58 |
R: 5´- CAG TCG GTC CAA AAT TCT TGT GA -3´ | |||
GABAb1 | F: 5´- ACG TCA CCT CGG AAG GTT G -3´ | 107 | 57 |
R: 5´- CAC AGG CAG GAA ATT GAT GGC -3´ | |||
5-HT1A | F: 5´- TGG GCA ATC ACC GAC CCT AT -3´ | 179 | 55.5 |
R: 5´- TAC CCG TGG TCC TTG CTG AT -3´ |