Fig. 3.

Histological photomicrographs of a native urinary bladder and reconstructed bladder at day 14 and day 90 post-implantation (hematoxylin and eosin staining). A Native urinary bladder wall showing the urothelium (U), submucosa (S) and muscular layer (M). B Reconstructed bladder at day 14 post-implantation showing muscle tissue ingrowth (white arrow) within the PLGA scaffold (black arrow). C Muscular layer of the reconstructed urinary bladder at day 90 post-implantation with aligned and elongated cells. D Newly reconstructed bladder wall showing complete regeneration of the urothelium (U), submucosa (S) and muscular layer (M). Scale bar: 100 μm for A, C, D; Scale bar: 50 μm for B