Table 2.

Box–Behnken experimental design matrix and experimental responses. Box–Behnken methodology represent 27 experiments based on different concentration (g/L) of four variables

Run	A(insulin) (g/L)	B(transferrin) (g/L)	C(selenium) (g/L)	D(glucose) (g/L)	R1(rhGM-CSF) (g/L)
1	0	1	0	-1	2.1
2	0	0	0	0	3.5
3	0	1	-1	0	2.2
4	-1	0	0	1	2.1
5	0	-1	0	1	2.4
6	1	0	1	0	2.7
7	0	-1	-1	0	2
8	1	0	0	-1	1.9
9	0	0	-1	1	2.2
10	0	0	0	0	3.3
11	0	0	0	0	3.5
12	0	-1	1	0	2.2
13	1	1	0	0	2.6
14	1	0	0	1	2.5
15	-1	-1	0	0	1.8
16	0	1	1	0	2.5
17	0	0	-1	-1	1.7
18	0	0	1	1	2.5
19	1	-1	0	0	2.4
20	1	0	-1	0	2.4
21	-1	1	0	0	2.1
22	0	1	0	1	2.7
23	0	-1	0	-1	1.7
24	-1	0	-1	0	1.8
25	0	0	1	-1	2
26	-1	0	0	-1	1.5
27	-1	0	1	0	2