Table 3.

Analysis of variance (ANOVA) for Response Surface Quadratic Model

Source	Sum of Squares	df	Mean Square	F-value	P -value
Model	6.75	14	0.4821	44.79	< 0.0001
A-A(insulin)	0.8533	1	0.8533	79.28	< 0.0001
B-B(transferrin)	0.2408	1	0.2408	22.37	0.0005
C-C(selenium)	0.2133	1	0.2133	19.82	0.0008
D-D(glucose)	1.02	1	1.02	94.84	< 0.0001
AB	0.0025	1	0.0025	0.2323	0.6385
AC	0.0025	1	0.0025	0.2323	0.6385
AD	0.0000	1	0.0000	0.0000	1.0000
BC	0.0025	1	0.0025	0.2323	0.6385
BD	0.0025	1	0.0025	0.2323	0.6385
CD	0.0000	1	0.0000	0.0000	1.0000
A²	2.31	1	2.31	214.74	< 0.0001
B²	1.59	1	1.59	147.62	< 0.0001
C²	1.97	1	1.97	183.36	< 0.0001
D²	2.77	1	2.77	257.45	< 0.0001
Residual	0.1292	12	0.0108
Lack of Fit	0.1025	10	0.0103	0.7687	0.6853
Pure Error	0.0267	2	0.0133
Cor Total	6.88	26