Fig. 6. Comparison of the NRC4 clade 53Kb deletion and the gain of function slnrc4a mutant.

Defense responses elicited by the MAMP EIX and disease susceptibility to B. cinerea were compared between the gain of function slnrc4a mutant and the recently reported 53 Kb near-full NRC4 clade genomic deletion. a ROS production was measured every 5 min for 50 min in WT M82, slnrc4a, WT GW, and GW∆53Kb lines using the HRP-luminol method. Average ± SEM of five independent replicates is shown, N > 48 per genotype. Results were analyzed for statistical significance in a two-way ANOVA, (p < 0.0001). Inset: total ROS production presented in a. Average ± SEM of five independent replicates is shown, asterisks represent statistically significant differences in a one-way ANOVA (p < 0.0001) with a Bonferroni post hoc test (*p-value < 0.05; ***p-value < 0.001). b Ethylene production of WT M82, slnrc4a, WT GW and GW∆53Kb samples was measured using gas chromatography. M82 or GW-WT average ethylene production is defined as 1. Average ± SEM of three independent experiments is presented, N > 10 per genotype. Asterisks represent statistical significance in one-way ANOVA (p < 0.0001) with a Dunnett post hoc test (****p < 0.0001; *p < 0.05). c Lesion area was measured seven days after inoculation with B. cinerea (10X⁶ spores/mL). Average ± SEM of three experiments (N > 24 per genotype). Asterisks represent statistical significant in a one-way ANOVA with a Tukey post-hoc test, p < 0.0001. ***p < 0.001, ****p < 0.0001. Boxplots are shown with inter-quartile-ranges (box), medians (black line in box) and outer quartile whiskers, minimum to maximum values.