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. 2020 Jul 30;10:12881. doi: 10.1038/s41598-020-69736-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Canstatin prevents interstitial fibrosis in non-infarcted area after MI. Recombinant mouse canstatin (20 μg/kg) or vehicle was intraperitoneally administered for 28 days after LAD ligation. (A, B) The hearts were isolated and then thin paraffin Sects. (4 μm) were made. (A) Representative picrosirius red stained pictures of the non-infarcted areas from SHAM + vehicle, SHAM + canstatin, MI + vehicle and MI + canstatin groups were shown. Scale bar: 100 μm. (B) Interstitial fibrotic area/total area was measured and shown as mean ± S.E.M. (SHAM + vehicle and SHAM + canstatin: n = 6; MI + vehicle and MI + canstatin: n = 8). (C) The tissue protein of non-infarcted area was extracted. Expression of type I collagen was detected by Western blotting. (Upper) Representative blots of type I collagen and vinculin were shown. (Lower) Levels of type I collagen were corrected by vinculin and the normalized expression relative to SHAM + vehicle was shown as mean ± S.E.M. (SHAM + vehicle and SHAM + canstatin: n = 6; MI + vehicle and MI + canstatin: n = 8). *, **P < 0.05, 0.01 vs. SHAM + vehicle, #, ## P < 0.05, 0.01 vs. MI + vehicle (two-way ANOVA followed by Tukey’s post hoc test).